You can also find the calibrated scale, this time in pixels/micron, by going to Analyze -> Set Scale. You do no need to make any changes here, select OK or close the window to exit. This will open a display window showing the dimensions of the image and the scale will be automatically populated at the correct size.įor this image we see the scale is automatically set at 2.2microns/pixel. In a calibrated image you can find the scale in microns/pixel by going to Image -> Properties (or use shortcut Ctrl+Shift+P). If the scale is already calibrated the dimensions shown on the image will be in microns instead of pixels. This is usually the case for images originally saved in the raw file format.
In some circumstances FIJI will be able to calibrate your image automatically by reading the attached metadata. Finding Scale Information in the Image Properties This section uses the images Interstitium 2.tif and TrichromeIHC.tif for demonstration.
#Imagej calibrate how to#
We will also demonstrate how to add a scale bar and how to crop and resize images without altering the image data. In this section we will show you how to find calibration information from the image, and how to set the scale if the image is not calibrated. Calibration is also important when resizing your images. Calibrating an image is also important if you want to get meaningful size-related measurements out of the image in any later analysis. This allows the viewer to see the scale of the image being presented and make their own conclusions about the data. For proper presentation of images it is essential to include a scale bar.
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